Tangential flow filtration for biologics purification
Continuous and gentle recirculation from 5 to 144 ml/min for TFF processes
Tangential flow filtration (TFF) is an established method for the purification and concentration of biologics. The technology is widely used in the production of monoclonal antibodies, vaccines, enzymes, and other biopharmaceutical products.
Unlike conventional filtration, the process fluid flows parallel to the membrane surface. This tangential flow continuously removes deposited particles from the membrane and helps reduce membrane fouling. While smaller molecules and process-related impurities pass through the membrane as permeate, the desired product remains in the retentate and becomes increasingly concentrated.
Recirculation of the process fluid requires precise fluid handling technology. Gentle pumping and stable flow rates are essential to protect sensitive biomolecules and maintain consistent process conditions throughout the filtration process.
What requirements does TFF place on fluid handling systems?
The purification of biologics requires a fluidic solution that combines product protection, process stability, and suitability for pharmaceutical environments.
Key requirements include:
- continuous recirculation of the retentate
- flow rates from 5 to 144 ml/min
- gentle handling of sensitive biomolecules
- stable differential pressures up to 5 bar
- minimal interaction with the product
- suitability for pharmaceutical process environments
Particularly in the processing of antibodies, enzymes, and other biologics, pumping technology plays an important role in product yield and product quality.
How is recirculation implemented in TFF systems?
The system utilizes the micro annular gear pump mzr-6355 from the hermetic inert series. It enables continuous and precise recirculation within the TFF loop.
For pharmaceutical applications, wetted components are available with a surface roughness of ≤ 0.51 Ra and FFPM elastomers supplied with material certificates. This allows the pump to be integrated into demanding pharmaceutical and biotechnology systems.
Multiple pumps are typically integrated into a single instrument or processing unit. Compact design supports space-saving OEM integration.
The pump is suitable for aqueous solutions, solvents, and cleaning solutions such as 0.1 M to 0.5 M sodium hydroxide (NaOH).
What benefits does this provide for TFF systems in pharmaceutical and biotechnology applications?
By integrating the micro annular gear pump, the TFF system can:
- gently recirculate biologics
- concentrate monoclonal antibodies, enzymes, and vaccines
- support stable filtration conditions
- operate membrane filtration processes reliably
- meet pharmaceutical material requirements
- enable compact OEM system designs
This fluidic solution supports the gentle purification and concentration of biologics in modern TFF systems for pharmaceutical and biotechnology applications.