Constant sheath flow in flow cytometry

Flow cytometry is a highly accurate measurement method for the analysis and quantification of cells and particles. These are transferred into a microchannel by means of a sheath flow and moved "single file" through the measuring area. The sheath flow must be continuous and the velocity constant to exclude measurement errors.

Micro annular gear pumps of the low-pressure series are used both for creating the sheath flow in the range from 1 to 10 ml/min and for cell and particle suspensions. Low-pulsation liquid flows and low-shear pumping are important prerequisites for use in this field of application.

Flow cytometry is used, among other fields, in hematology, infectiology; immunology and basic medical and cell biology research. One biotechnological application is the sorting of sperm cells according to sex chromosomes, another is the differentiation of living and dead cells.


Decision factors for the pumps

  • continuous, smooth volume flow (low pulsation)
  • low-shear transport of cells and particles
  • high precision
  • high repeatability
  • long service life


Applied pumps

 

< Back to overview